polyclonal anti-calr antibody  (Swant)

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

doi: 10.14715/cmb/2024.70.1.14

Figure Lengend Snippet: Fig. 5. The interaction of TMCO1 with CALR and VDAC1 and its influence on the expression of EMT markers. (A-H) Western blotting was conducted for examining (B) TMCO1, (C) VDAC1, (D) CALR, (E) Vimentin, (F) β-catenin, (G) N-cadherin, and (H) E-cadherin ex pression in SK-OV-3 cells subjected to TMCO1-knockout or overex pressed plasmids. (I-P) Western blotting was conducted to examine (J) TMCO1, (K) VDAC1, (L) CALR, (M) Vimentin, (N) β-catenin, (O) N-cadherin, and (P) E-cadherin expression in SK-OV-3-CDDP cell line subjected to TMCO1-knockout or overexpressed plasmids. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Article Snippet: Thereafter, the cells were sealed by 1% BSA for 1 hour at room temperature, as well as incubated by primary antibodies against TMCO1 (1:200; ab220729; Abcam, Cambridge, MA, USA), β-catenin (1:250; ab184919; Abcam, Cambridge, MA, USA), Vimentin (1:200; 10366-1-AP; Proteintech, Rosemont, IL, USA), N-cadherin (1:100; 22018- 1-AP; Proteintech, Rosemont, IL, USA), VDAC1 (1:100; 55259-1-AP; Proteintech, Rosemont, IL, USA), CALR (1:200; 27298-1-AP; Proteintech, Rosemont, IL, USA) and E-cadherin (1:150; ab231303; Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Expressing, Western Blot, Knock-Out

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

doi: 10.14715/cmb/2024.70.1.14

Figure Lengend Snippet: Fig. 6. The interaction of TMCO1 with CALR and VDAC1 and its influence on the expression of EMT markers. (A, B) Representative images of immunofluorescence for SK-OV-3 or SK-OV-3-CDDP cell lines. Bar scale, 20 μm. (C-G) The expression levels of (C) TMCO1, (D) CALR, (E) VDAC1, (F) N-cadherin, and (G) E-cadherin were quantified in SK-OV-3 cells subjected to TMCO1-knockout or ove rexpressed plasmids. (H-L) The expression levels of (H) TMCO1, (I) CALR, (J) VDAC1, (K) N-cadherin, or (L) E-cadherin were quanti fied for SK-OV-3-CDDP cell line with TMCO1-knockout or overex pressed plasmids. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Article Snippet: Thereafter, the cells were sealed by 1% BSA for 1 hour at room temperature, as well as incubated by primary antibodies against TMCO1 (1:200; ab220729; Abcam, Cambridge, MA, USA), β-catenin (1:250; ab184919; Abcam, Cambridge, MA, USA), Vimentin (1:200; 10366-1-AP; Proteintech, Rosemont, IL, USA), N-cadherin (1:100; 22018- 1-AP; Proteintech, Rosemont, IL, USA), VDAC1 (1:100; 55259-1-AP; Proteintech, Rosemont, IL, USA), CALR (1:200; 27298-1-AP; Proteintech, Rosemont, IL, USA) and E-cadherin (1:150; ab231303; Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Expressing, Immunofluorescence, Knock-Out

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

doi: 10.14715/cmb/2024.70.1.14

Figure Lengend Snippet: Fig. 7. The interaction of TMCO1 with CALR and VDAC1. (A-L) Western blotting was conducted to examine (B) TMCO1, (C)CALR and( D) VDAC1 expression with CALR-siRNA and VDAC1-siRNA transfections. (E-H) Western blotting was conducted for examining (F) TMCO1, (G) CALR, (H) VDAC1 in SK-OV-3 cells. (I-L) Wes tern blotting was conducted for examining (J) TMCO1, (K) CALR, (L) VDAC1 With TMCO1 overexpression or CALR-siRNA and VDAC1-siRNA transfections in SK-OV-3-CDDP cells.

Article Snippet: Thereafter, the cells were sealed by 1% BSA for 1 hour at room temperature, as well as incubated by primary antibodies against TMCO1 (1:200; ab220729; Abcam, Cambridge, MA, USA), β-catenin (1:250; ab184919; Abcam, Cambridge, MA, USA), Vimentin (1:200; 10366-1-AP; Proteintech, Rosemont, IL, USA), N-cadherin (1:100; 22018- 1-AP; Proteintech, Rosemont, IL, USA), VDAC1 (1:100; 55259-1-AP; Proteintech, Rosemont, IL, USA), CALR (1:200; 27298-1-AP; Proteintech, Rosemont, IL, USA) and E-cadherin (1:150; ab231303; Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Western Blot, Expressing, Transfection, Over Expression

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

doi: 10.14715/cmb/2024.70.1.14

Figure Lengend Snippet: Fig. 8. The interaction of TMCO1 with CALR and VDAC1. (A-D) Western blotting was conducted for examining (B) TMCO1, (C) CALR and (D) VDAC1 expression with CALR-siRNA +VDAC1- siRNA or sh-TMCO1 with CALR-siRNA and VDAC1-siRN in SK- OV-3 cells. (E-H) Western blotting was conducted for examining (F) TMCO1, (G) CALR and (H)VDAC1 expression with CALR-siRNA +VDAC1-siRNA or sh-TMCO1 with CALR-siRNA and VDAC1- siRNA in SK-OV-3-CDDP cells.

Article Snippet: Thereafter, the cells were sealed by 1% BSA for 1 hour at room temperature, as well as incubated by primary antibodies against TMCO1 (1:200; ab220729; Abcam, Cambridge, MA, USA), β-catenin (1:250; ab184919; Abcam, Cambridge, MA, USA), Vimentin (1:200; 10366-1-AP; Proteintech, Rosemont, IL, USA), N-cadherin (1:100; 22018- 1-AP; Proteintech, Rosemont, IL, USA), VDAC1 (1:100; 55259-1-AP; Proteintech, Rosemont, IL, USA), CALR (1:200; 27298-1-AP; Proteintech, Rosemont, IL, USA) and E-cadherin (1:150; ab231303; Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Western Blot, Expressing

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

doi: 10.14715/cmb/2024.70.1.14

Figure Lengend Snippet: Fig. 10. Targeting TMCO1 inhibited the expression of CALR, VDAC1 and EMT markers in xenograft tumors. (A-K) Western blot was ap plied for examining (B) TMCO1, (C) VDAC1, (D) CALR, (E) Ki-67, (F) MMP2, (G) MMP9, (H) N-cadherin, (I) Vimentin, (J) β-catenin, or (K) E-cadherin expression within xenograft tumors developed by sh-NC or sh-TMCO1-transfected SK-OV-3 or SK-OV-3-CDDP cell line. **P<0.05; ***P<0.001; ****P<0.0001.

Article Snippet: Thereafter, the cells were sealed by 1% BSA for 1 hour at room temperature, as well as incubated by primary antibodies against TMCO1 (1:200; ab220729; Abcam, Cambridge, MA, USA), β-catenin (1:250; ab184919; Abcam, Cambridge, MA, USA), Vimentin (1:200; 10366-1-AP; Proteintech, Rosemont, IL, USA), N-cadherin (1:100; 22018- 1-AP; Proteintech, Rosemont, IL, USA), VDAC1 (1:100; 55259-1-AP; Proteintech, Rosemont, IL, USA), CALR (1:200; 27298-1-AP; Proteintech, Rosemont, IL, USA) and E-cadherin (1:150; ab231303; Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Expressing, Western Blot, Transfection

Journal: Cellular and molecular biology (Noisy-le-Grand, France)

Article Title: TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

doi: 10.14715/cmb/2024.70.1.14

Figure Lengend Snippet: Fig. 11. The role of TMCO1, CALR, and VDAC1 in xenograft tu mors. (A, B) Representative images of (A) immunohistochemistry as well as (B) immunofluorescence in xenograft tumors. Bar scale, 20 μm. (C-I) Expression of (C) TMCO1, (D) VDAC1, (E) CALR, (F) β-catenin, (G) Vimentin, (H) N-cadherin, or (I) E-cadherin was quantified in xenograft tumors developed by sh-NC or sh-TMCO1- transfected SK-OV-3 or SK-OV-3-CDDP cell line according to immu nohistochemistry. (J-P) Expression of (J) TMCO1, (K) VDAC1, (L) CALR, (M) β-catenin, (N) Vimentin, (O) N-cadherin, or (P) E-cadhe rin was quantified within xenograft tumors developed by sh-NC or sh- TMCO1-transfected SK-OV-3 or SK-OV-3-CDDP cell line according to immunofluorescence. **P<0.05; ***P<0.001; ****P<0.0001.

Article Snippet: Thereafter, the cells were sealed by 1% BSA for 1 hour at room temperature, as well as incubated by primary antibodies against TMCO1 (1:200; ab220729; Abcam, Cambridge, MA, USA), β-catenin (1:250; ab184919; Abcam, Cambridge, MA, USA), Vimentin (1:200; 10366-1-AP; Proteintech, Rosemont, IL, USA), N-cadherin (1:100; 22018- 1-AP; Proteintech, Rosemont, IL, USA), VDAC1 (1:100; 55259-1-AP; Proteintech, Rosemont, IL, USA), CALR (1:200; 27298-1-AP; Proteintech, Rosemont, IL, USA) and E-cadherin (1:150; ab231303; Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Immunohistochemistry, Immunofluorescence, Expressing, Transfection

Journal: Cell reports

Article Title: Disruption of the lipolysis pathway results in stem cell death through a sterile immunity-like pathway in adult Drosophila

doi: 10.1016/j.celrep.2022.110958

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following antibodies were used: rabbit polyclonal anti-BiP (1:200; Novus Biologicals, Cat # NBP1–06274); guinea pig polyclonal anti-Mcr (1:200; from Robert Ward); rabbit polyclonal anti-Calr (1:1000; Abcam, Cat # ab2907); rabbit polyclonal anti-LRP1 (1:1000; from Suzanne Eaton); mouse monoclonal anti-Drpr (1:100; DSHB 8A1); mouse monoclonal anti-Prtp (1:100; generated in our laboratory); rabbit polyclonal anti-peIF2α (1:1000; Cell Signaling, Cat # 9721S); rabbit polyclonal anti-Ref2P (Abcam, Cat # ab178440); rabbit polyclonal anti-HA tag (Cat # ab9110); rabbit polyclonal anti-GFP (1:500, Thermo Fischer Scientific, Cat # A6455); and mouse monoclonal anti-GFP (1:100; Thermo Fischer Scientific, Cat# A11120).

Techniques: Recombinant, Plasmid Preparation, Electron Microscopy, Software